Study on mechanism of Klotho reversing resistance of breast cancer to paclitaxel by inhibiting autophagy
10.11659/jjssx.09E017007
- VernacularTitle:Klotho通过抑制自噬逆转乳腺癌紫杉醇耐药的作用机制研究
- Author:
Fang-Yi LONG
1
;
Ping JIA
;
Hua-Fei WANG
;
Yi QING
;
Ting-Ting XIONG
;
Meng-Jie HE
;
Jin SUN
;
Fang CHEN
;
Qian-Ru SUN
;
Ting WANG
Author Information
1. 四川省妇幼保健院药学部
- Keywords:
klotho;
autophagy;
breast cancer;
paclitaxel;
drug resistance
- From:Journal of Regional Anatomy and Operative Surgery
2017;26(11):796-799
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of klotho reversing the resistance of breast cancer to paclitaxel in MCF-7/PTX cells.Methods The Klotho expression in MCF-7 and MCF-7/PTX cells was detected by Western blot.The effects of Klotho on paclitaxel resistance in MCF-7/PTX cells was measured by MTT assay.The effects of Klotho and 3-methyladenine (3-MA) on proliferation and expression of Beclin1 in MCF-7/PTX cells were detected by MTT and Western blot assay,respectively.Results The expression of Klotho in MCF-7/PTX cells was decreased compared with MCF-7 cells.Klotho could sensitize MCF-7/PTX cells to paclitaxel.The expression of Beclin1 in MCF-7/PTX cells was higher than that in MCF-7 cells.Klotho and 3-MA could decrease the expression of Beclin1 in MCF-7/PTX cells,and the effects of Klotho on paclitaxel resistance in MCF-7/PTX cells was similar to that of 3-MA.Conclusion Paclitaxel resistance in breast cancer cells is related to expression of the Klotho which can reverse the resistance of breast cancer to paclitaxel by inhibiting autophagy.
