Role of oxytocin in neonatal rat hippocampal neurons after hypoxic-ische-mic injury
10.3969/j.issn.1000-4718.2018.04.026
- VernacularTitle:催产素减轻新生大鼠海马神经元缺氧缺血性损伤
- Author:
Chang-Ning XIE
1
;
Jian WU
;
Xin-Meng WANG
;
Si-Cong PENG
;
Jing WU
;
Ling-Hui XIAO
;
Tao LIU
Author Information
1. 南昌大学第一附属医院儿科
- Keywords:
Oxytocin;
Hypoxic-ischemic encephalopathy;
Hippocampus;
Patch-clamp techniques
- From:
Chinese Journal of Pathophysiology
2018;34(4):739-744,753
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether oxytocin has neuroprotective effects on hippocampal CA 1 pyramidal neurons from neonatal rats exposed to hypoxic-ischemic brain injury and the underlying mechanisms.METHODS:An in vitro model of hypoxic-ischemic injury was used by exposing the brain slices to oxygen-glucose deprivation(OGD)solution. Acute dissociated brain slices(6~8 slices per rat)from 8 Sprague-Dawely rats of 7~10 d old were used.The slices were randomly divided into 4 groups: control group, OGD 20 min group, OGD 40 min group and OGD +oxytocin group.The effect of oxytocin on neuronal death was evaluated by TO-PRO-3 staining.Fresh brain slices from other 20 neonatal rats were divided into OGD group,OGD+oxytocin group,OGD+dVOT(oxytocin receptor antagonist)+oxytocin group,and OGD+bicucuclline(GABAAreceptor antagonist)+oxytocin group.The onset of anoxic depolarization in the hippocampal neurons treated with different drugs was recorded by whole-cell patch-clamp techniques.RESULTS: The results of TO-PRO-3 staining showed that neuronal deaths in hippocampal CA 1 area were increased over the prolonged OGD time.Oxyto-cin significantly reduced the hypoxic-ischemic deaths.Oxytocin dramatically prolonged the onset time of anoxic depolariza-tion after the application of OGD solution.Both dVOT and bicuculline blocked this effect.CONCLUSION: Oxytocin plays a neuroprotective role in neonatal rat hippocampal CA 1 pyramidal neurons by enhancing the inhibitory synaptic trans-mission via oxytocin receptors.Therefore,oxytocin is useful as a candidate for neuroprotective treatment after neonatal hy -poxic-ischemic brain injury.
