Effect of miR-153-targeted PRDM2 on invasion and migration abilities of bladder cancer cells by JAK/STAT signaling pathway
10.3969/j.issn.1000-4718.2018.01.010
- VernacularTitle:miR-153靶向PRDM2基因并通过JAK/STAT信号通路影响膀胱癌的侵袭和迁移
- Author:
Guan-Jun LI
1
;
Guo-Wei YA
;
Zheng-Yan TANG
;
Kai-De SU
Author Information
1. 南阳医学高等专科学校第一附属医院 泌尿外科
- Keywords:
Bladder cancer;
MicroRNA-153;
Positive regulatory domain zinc finger protein 2;
JAK/STAT signaling pathway
- From:Chinese Journal of Pathophysiology
2018;34(1):58-63
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of microRNA(miR)-153-targeted positive regulatory domain zinc finger protein 2(PRDM2 )on invasion and migration abilities of bladder cancer cells through JAK /STAT signaling path-way.METHODS:The expression levels of miR-153 in bladder cancer tissues were detected by qPCR.The expression of PRDM2 in normal tissues and bladder cancer tissues was detected by immunohistochemistry.The effect of miR-153 on the transcriptional activity of PRDM2 was examined by dual-luciferase reporter assay system.The effect of miR-153 over-ex-pression on the invasion ability of bladder cancer RT 4 cells was detected by Transwell assay ,and the cell invasion ability was analyzed by scratch test.The protein levels of PRDM2,p-JAK2 and p-STAT3 were determined by Western blot.RE-SULTS:The expression of miR-153 was significantly lower in the bladder cancer tissues than that in normal tissues(P<0.05).PRDM2 was highly expressed in the bladder cancer tissue(P<0.05).The results of dual-luciferase reporter assay system showed that miR-153 regulated the expression of PRDM2.miR-153 over-expression significantly decreased the invasion and migration abilities of bladder cancer RT 4 cells.miR-153 over-expression also down-regulated the protein levels of p-JAK2 and p-STAT3(P<0.05).CONCLUSION:miR-153 targets PRDM2,and regulates the invasion and migra-tion abilities of bladder cancer cells by JAK/STAT signaling pathway.
