Optimization on Transformation from Chlorogenic Acid to Neochlorogenic Acid by Box-Behnken Design-response Surface Methodology
- VernacularTitle:Box-Behnken Design-响应面法优化绿原酸转化为新绿原酸的工艺研究
- Author:
Jianhui WEN
1
;
Fuyong NI
;
Xuejing WANG
;
Ming LI
;
Yaling SONG
;
Zhenzhong WANG
;
Wei XIAO
Author Information
- Keywords: Chlorogenic acid; neochlorogenic acid; Box-Behnken design-response surface methodology; structural identification
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(7):1239-1244
- CountryChina
- Language:Chinese
- Abstract: Box-Behnken design-response surface methodology was used to optimize the transformation from chlorogenic acid to neochlorogenic acid.Based on single factor experiments,the experiment design were developed by box-benhnhen central composite design with causal factors of the reaction temperature,time and PH to neochlorogenic acid.The optimum transformation conditions were as follow:reaction temperature at 107℃,reaction time of 60 min,the PH of 4.72.Under the optimum extraction technology conditions,the productivity of neochlorogenic acid was 64.20％.Neochlorogenic acid was isolated and purified.The determination and characterization of neochlorogenic acid was detected by HPLC,1H-NMR,13C-NMR and ESI-MS.The results showed that the content of neochlorogenic acid reached to 98.78％ and the yield of 87.37％.
