Experimental Study on Inhibition Effect of Berberine in Escherichia coil Gene Transcription
- VernacularTitle:小檗碱对大肠杆菌基因转录抑制作用的实验研究
- Author:
Huiyu LI
1
;
Yugang WANG
;
Zhiyi YUAN
;
Fan LEI
;
Xinpei WANG
;
Xi LU
;
Dongming XING
;
Jun LI
;
Lijun DU
Author Information
- Keywords: Berberine; Escherichia coli; gene transcription; TATA box
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(4):569-577
- CountryChina
- Language:Chinese
- Abstract: There have been many reports on berberine (BBR) effect of the inhibition on gut bacteria,but more from the protein level.In view of the preference of BBR for DNA binding,we here investigated the expression of BBR from the transcriptional expression level of the gene.The results showed that BBR had a higher affinity for UP element of Escherichia coli (E.coli) gene,and the transcription initiation region of this element contained TATA base sequence.The expression of genes sulA,recA and 16S which contain the genes of the UP element regulatory elements in the upstream of the promoter could be suppressed by BBR,and the expression of lpxC,secG and mutT which did not contain the genes of the UP element regulatory elements in the upstream of the promoter could not be inhibited by BBR.It is shown that the TATA sequence is the target of BBR.This result provides a new perspective for exploring the effect of BBR's inhibition of microbiota from gene transcription.
