Effects of propofol sedation on BDNF-TrkB/p75 signal and cognitive function in rat hippocampus
10.3969/j.issn.1674-8115.2018.06.002?
- VernacularTitle:丙泊酚镇静对大鼠海马BDNF-TrkB/p75信号和认知功能的影响
- Author:
Wen-Juan YU
1
;
Min ZHU
;
Yan WO
;
Yi-Min YU
;
Yan LI
;
Hong-Wei FANG
;
Hao ZHU
Author Information
1. 上海交通大学 医学院附属精神卫生中心
- Keywords:
propofol;
brain derived neurotropic factor;
hippocampus;
sedation
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2018;38(6):594-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective·To detect the effects of propofol sedation on cognitive function in rats and its mechanism. Methods?·?Forty-eight SD rats were randomly divided into three groups, i.e. control group, 100?mg/kg group and 300?mg/kg group. Rats were administrated intraperitoneally with propofol (10?mg/mL, 100?mg/kg or 300?mg/kg). The mRNA levels of brain derived neurotropic factor (BDNF)-TrkB/p75 signal molecules in rat hippocampus were evaluated by realtime PCR 45 min after propofol treatment. Learning and memory ability was examined by inhibitory avoidance (IA) test after propofol treatment. Results?·?The mRNA levels of BDNF in the hippocampal tissue were (1.20±0.13) fold (P=0.002) and (88±12) % (P=0.044) of that in control group, respectively, in 100?mg/kg group and 300?mg/kg group after injection of propofol. The mRNA levels of TrkB were (1.01±0.11) fold ( P=0.982) and (86±11) % (P=0.018) of that in control group, respectively, in 100?mg/kg group and 300?mg/kg group. The mRNA levels of p75 were (1.02±0.10) fold (P=0.778) and (1.59±0.18) fold (P=0.000) of that in control group, respectively, in 100?mg/kg group and 300?mg/kg group. There was no significant difference of the 24 h IA memory retention latency between 100?mg/kg group and control group. The 24 h IA memory retention latency in 300?mg/kg group was significantly decreased compared with control group (P=0.028) and 100?mg/kg group (P=0.020). Conclusion?·?Propofol dose-dependently regulates the expression of BDNF-TrkB/p75 signal molecules, and high dose propofol may reduce cognitive function via BDNF-TrkB/p75 signal.
