The research of chlorogenic acid content in Wudang Lonicerae Flos (Ⅰ)by different drying technology
10.3760/cma.j.issn.1673-4246.2018.09.017
- VernacularTitle:不同干燥方法对武当一号山银花中绿原酸含量的影响
- Author:
Cong LI
1
;
Fang ZHENG
;
Yutao ZHOU
;
Zhihao LI
;
Huimin LIU
;
Lin XIONG
;
Lingjie HUANG
Author Information
1. 442000十堰,湖北医药学院附属东风医院药学部,武当特色中药研究所湖北省重点实验室
- Keywords:
Chromatography,high pressure liquid;
Flos Lonicerae;
Chlorogenic acid;
Quality control
- From:International Journal of Traditional Chinese Medicine
2018;40(9):861-864
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the different drying technology.on Wudang Lonicerae Flos (Ⅰ) in chlorogenic acid content.Methods Using HPLC method to research the difference in the contents of chlorogenic acid of Wudang Lonicerae Flos (Ⅰ) employing oven-drying method,air-dried method and steamed drying technologies.DIONEX C18 (4.6 mm×250 mm,5 μm) was used.The mobile phases consisted of acetonitrile-0.4％ phosphoric acid aqueous solution (17 ∶ 83).The detection wavelengths of chlorogenic acid was 327nm.The flow rate was 0.4 ml/min.The column temperature was 30 ℃.Results The Separation of Chlorogenic acid content was satisfactory.The concentration and peak area showed a good linearity relationship with the range of 0.285-2.850 μg.Precision,repeatability and stability met the requirements.The average recovery was 100.26％ with RSD 1.95％.Chlorogenic acid content in the steamed drying Wudang Honeysuckle (Ⅰ) was higher.Conclusions The method of steam drying is better than that of oven-drying method and drying.
