Determination of quercetin, luteolin and apigenin in Matricaria Chamomila L. by PR-HPLC
10.3760/cma.j.issn.1673-4246.2018.07.016
- VernacularTitle:HPLC法测定德国洋甘菊中槲皮素、木犀草素及芹菜素含量
- Author:
Xin LENG
1
;
Jian NI
;
Longtai YOU
;
Xiaoxu DONG
;
Meiling WANG
;
Xingbin YIN
Author Information
1. 100029,北京中医药大学中药学院
- Keywords:
Chromatography,high pressure liquid;
Matricaria Chamomila L.;
Quercetin;
Luteolin;
Apigenin;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2018;40(7):649-652
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for simultaneous determination of Quercetin , Luteolin, pigenin in Matricaria Chamomila L.. Methods HPLC analysis was performed on Agilent Zorbox SB-C18 column (4.6 mm×250 mm, 5 μm) with the methanol and phosphoric acid as mobile phase in equal degree model, and the column temperature was set at 25 ℃, and the flow rate was 1.0 ml.min-1 and the detecting wave length was at 350 nm. Results The linear response ranges were from 0.25-4.04 μg of Quercetin (r=1.000, n=6) and from 0.25-3.98 μg of Luteolin (r=1.000, n=6) and 0.25-4.02 μg of Apigenin (r=1.000, n=6);and the recoveries, the precision and the stability RSD of Quercetin, Luteolin and Apigenin meet the requirements. The average recovery rate of quercetin, luteolin and apigenin was 93.64%, 95.85% and 95.40%, respectively. Conclusions All 3 samples in Matricaria Chamomila.L were determined by HPLC. The method is simple, rapid, and reproducible. It can be used as reference for the quality control of Matricaria Chamomila L..
