Effects of Celastrol on Accelerated Healing of Skin Wounds of Diabetic Ulceration in Rats and Mechanism Revealed by Nuclear Magnetic Resonance-based Metabonomics
10.11895/j.issn.0253-3820.170323
- VernacularTitle:核磁共振代谢组学方法研究雷公藤红素对大鼠糖尿病溃疡促愈合作用机制
- Author:
Yong-Sheng HU
1
;
Peng-Tao XU
;
Sheng-Jie YE
;
Wen-Yu SHI
;
Hong-Chang GAO
;
Yang ZHUO
;
Liang-Cai ZHAO
Author Information
1. 解放军第118医院
- Keywords:
Celastrol;
Diabetic ulceration;
Nuclear magnetic resonance;
Metabolomics
- From:
Chinese Journal of Analytical Chemistry
2018;46(2):170-177
- CountryChina
- Language:Chinese
-
Abstract:
The experimental SD rats were randomly divided into normal control group (Con group),diabetic ulcer model group (DM group) and Celastrol group (Cel group).Except the control group,diabetic ulceration rat models were established by intraperitoneal injection of streptozotocin along with skin scald.And then,each group was treated by spraying the saline solution on the affected skin with (Cel group) or without (Con group and DM group) Cel (q.d.×14 d).Nuclear magnetic resonance (NMR)-based metabonomic analysis was applied to detect metabolic characteristics,accompanied by healing rate calculation and HE and Masson staining to study therapeutic effect of celastrol on accelerated healing of skin wounds of diabetic ulceration rats,which could be used to elucidate therapeutic effects of celastrol on the rat diabetic ulceration and its mechanism.The results showed that celastrol could induce epithelial regeneration of the rat ulcer wound,regulate the infiltration of inflammatory cells and the distribution of collagen fibers,and promote the healing of the ulcer wound.About 20 endogenous potential differential metabolites were screened and identified by partial least square analysis.Metabolic pathway analysis was carried out to show that celastrol can significantly recovery the level of the tricarboxylic acid cycle,promote its energy supply,accelerate the protein synthesis,improve mitochondrial dysfunction and oxidative stress,and accelerate the self-repair ability of skin tissue.Celastrol can promote the healing of ulcers skins of the diabetic rats,which contribute to experimental basis of the drugs for the treatment of diabetic ulcers.
