Proteomics study of rapamycin-injured human coronary artery endothelial cells based on two dimension fluorescence differential gel electrophoresis
10.3969/j.issn.1671-8348.2018.08.001
- VernacularTitle:基于荧光差异双向电泳的雷帕霉素损伤人冠状动脉内皮细胞的蛋白质组学研究
- Author:
Shaojun LIU
1
;
Kunhua HU
;
Yun ZHONG
Author Information
1. 广州医科大学附属第二医院心内科/广州心血管疾病研究所
- Keywords:
rapamycin;
eluting-stent-drug;
human coronary artery endothelial cells;
molecular mechanisms;
proteomic study
- From:
Chongqing Medicine
2018;47(8):1009-1011,1015
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanisms of the effect of eluting stent drug rapamycin for injuring human coronary artery endothelial cells(HCAECs)by using the proteomics method.Methods HCAECs were treated with rapamycin,and the differentially expressed proteins were analyzed by two dimension fluorescence differential gel electrophoresis(2D-DIGE).The changed proteins were identified by MALDI-ToF-ToF.Results At least 85 differential protein spots were found,including 49 up-regulated and 36 down-regulated protein spots.Twenty-six proteins were identified by MALDI-ToF-ToF,including the endoplasmic reticulum protein,mitochondrial protein,molecular chaperones,ubiquitin system related protein,structural protein and oxidative stress related proteins,etc.Conclusion The changes of specific proteins of HCAECs injury induced by rapamycin are investigated by the proteomic method.
