Preparation of hyaluronic acid decorated chlorogenic acid liposome and cytology study
10.3969/j.issn.1671-8348.2018.04.005
- VernacularTitle:透明质酸修饰的绿原酸脂质体的制备及细胞学研究
- Author:
Yuxi ZHAO
1
;
Fan ZHANG
;
Qin YANG
;
Ying WEI
;
Chuan CHEN
;
Fu LIU
Author Information
1. 川北医学院药学院药物研究所
- Keywords:
hyaluronic acid;
chlorogenic acid;
cell line,tumor;
target;
liposome
- From:
Chongqing Medicine
2018;47(4):449-452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare hyaluronic acid(HA) targeted chlorogenic acid(HA-CA) liposome and to investigate its inhibition effect on HA receptor(CD44) high expressing A549 cells and HA receptor(CD44) low expression HepG2 cells proliferation.Methods HA-DOPE was synthesized;HA-CA liposome was prepared by thin membrane disperse method and the particle size was measured by using the dynamic light scattering particle size analyzer;the HPLC method was adopted to establish the CA in vitro contents measurement method and detect the HA-CA liposome entrapment efficiency;MTT assay was applied to detect the proliferation inhibiting effect of free CA,CA liposome and HA-CA liposome on A549 cells and HepG2 cells;the fluorescence cell uptake assay was adopted to verify the targeting effect of HA liposome.Results The average particle size of HA-CA was 219.20 nm and PDI was 0.16;the entrapment efficiency of HA-CA liposome was(85.36 ± 1.01)％;the proliferation inhibition effect of HA-CA liposome on A549 cells was significantly greater than that of CA liposome,moreover CA liposomewas greater than free CA,the proliferation inhibition effect of CA liposome and HA-CA liposome on HepG2 cells was basically similar,which was greater than that of free CA;the uptake of A549 cells on HA liposome carrying 6-coumarin(HA-C liposome) was higher than that of HepG2.Conclusion HA-CA liposome can specifically combined with the high expression HA receptor cells to achieve the active targeting effect of tumor cells.
