Protective Effect of Riptolide on Renal Tissures in Type 2 Diabetic Rats
- VernacularTitle:雷公藤甲素对2型糖尿病大鼠肾脏的保护作用
- Author:
Rui-Xia MA
;
Li-Qiu LIU
;
Yan XU
;
Wei JIANG
;
- Publication Type:Journal Article
- Keywords:
Triptolide;
Diabetic nephropathy;
Osteopontin;
Monocyte chemoaltractant protein-1;
Macrophage
- From:
Chinese Journal of Hypertension
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the renal protective effects of triptolide treatment in type 2 diabetic rats and its possible mechanisms.Methods Wistar rats were randomized to receive following approach control (n=7),dia- betic model without treatment (n=7) and diabetic group treated with triptolide[200 ?g/(kg?d),n=7].Plasma glucose (PG),serum creatinine (Scr),total cholesterin (TC),triglyeride (TG),kidney mass (KM),index number of kidney hypertrophy (KM/body mass,KM/BM),and 24 hours urinary albumin (24 h UAL) were determined. The protein expression of monocyte chemoattractant protein-1 (MCP-1),osteopontin (OPN) and ED-1 in renal tissue were determined by immunohistochemical technique.The mRNA expressions of MCP-1 and OPN in kidney tissue were assessed by reverse transcription-polymerase chain reaction (RT-PCR).Results Elevated 24 h UAL was markedly attenuated by triptolide treatment [24 h UAL,triptolide:2.32?0.29 vs diabetic model group:3.89? 0.51 mg/mgCr,P
