Identification of seven plants of Gynostemma Bl.by ISSR-PCR
- VernacularTitle:ISSR-PCR鉴别绞股蓝属7种植物
- Author:
Chong WANG
;
Tian-Hua ZHOU
;
Xue YANG
;
Jing GUO
;
Gui-Fang ZHAO
;
- Publication Type:Journal Article
- Keywords:
Crynostemma Bl.;
ISSR;
DNA fingerprint;
classified identification
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify seven species of Gynostemma BI.,including G.pentaphyllum,G. pentagynum,G.cardiospermum,G.longipes,G.yixingense,G.laxiflorum,and G.guangxiense,by in- ter-simple sequence repeat(ISSR)markers.Methods General DNA was isolated from leaves of the seven species in Gynostemma B1.by CTAB,57 primers constituted by ISSR were tested for PCR and sepharose electrophoresis.Results Fourteen primers amplified polymorphic bands,the amplification patterns of primers UBC-873 and UBC-895 were higher in terms of polymorphic and amplified band ratio.They are used to distinguish all the examined seven species.Conclusion ISSR-PCR Method provides a quick,reli- able molecular marker technique for the identification of different species of Gynostemma B1.
