The experimental study of intratracheal administration of anti-tumor necrosis factor-? antibody attenuating lung injury after cardiopulmonary bypass
- VernacularTitle:抗肿瘤坏死因子抗体减轻体外循环的肺损伤
- Author:
Yang YU
;
Dan-Ni QI
;
Hua WEI
;
Hui HU
;
Xiao LIU
;
Chengxiong GU
;
- Publication Type:Journal Article
- Keywords:
Extracorporeal circulation;
Apoptosis;
Lung/injuries;
Anti-tumor necrosis factor-?antibody;
Lung protection
- From:Chinese Journal of Thoracic and Cardiovascular Surgery
1995;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect and its mechanism of anti-tumor necrosis factor-?antibody (TNF-? Ab) on lung injury after cardiopulmanary bypass(CPB).Methods Twenty-eight healthy rabbits were selected and randomly divided into four groups:group Ⅰ only received open chest operation;groups Ⅱ-Ⅳ underwent CPB.In the group Ⅳ,rabbit TNF-? Ab (2 400 pg/kg) was dropped into the intracheal tube before operation and just after releasing the aortic clamp.Saline was given to the group Ⅲ in- stead.Blood neutrophils count,TNF-?,MDA from the right and left atrium in the four groups were determined perioperatively.Water volume,TNF-? mRNA,TNF-? protein,apoptosis and pathomorphological changes were measured in the lung tissues.Results TNF- ? Ab can restrain leukocyte accumulation,reduce releasing of TNF-? and MDA in the lung.It can also reduce the occurrence of apop- tosis and attenuate pathomorphological changes in the lung tissue.However,it cannot reduce the secretion of TNF-? at the transcrip- tion level and protein level.Conclusion Intratracheal TNF-? Ab administration has markedly protective effect on lung injury after CPB.
