Effects on osteoblast and adipocyte differentiation of bone marrow mesenchymal stimulated by pulsed electromagnetic fields
- VernacularTitle:脉冲电磁场对骨髓间充质干细胞成骨成脂肪分化的影响
- Author:
Yong YANG
;
Hua WU
;
Dong-Ming ZHAO
;
Wen-Chun ZHAO
;
Run-Guang LI
;
- Publication Type:Journal Article
- Keywords:
Pulsed electromagnetic fields;
Osteocalcin;
Osteopontin;
Alkaline phosphatase;
Bone marrow mesenchymal stem cell
- From:Chinese Journal of Physical Medicine and Rehabilitation
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of pulsed electromagnetic fields(PEMFs)of 15Hz and lmT on osteoblast differentiation of bone marrow mesenchymal stem cells.Methods The bone marrow mesenchymal stem cells were isolated and cultured in vitro,the third passage cells were harvested and half of them was exposed to PEMFs,another half exposed to sham PEMFs served as the control.The activity of ALP was measured.And the ex- pression of osteoblast and adipogenesis was measured with the semi-quantitative RT-PCR,adipogenic differentiation was observed with the Oil Red O Staining.Results When compared with the control,PEMFs of 15 Hz and 1 mT significantly increased the activity of ALP(P
