Fffect of p38MAPK signal transduction pathway on RANTES expression after unilateral ureteral obstruction in rats
- VernacularTitle:p38信号通路对单侧输尿管结扎大鼠RANTES的影响
- Author:
Hua SHUI
;
Guohua DING
;
- Publication Type:Journal Article
- Keywords:
Renal interstitum;
p38 mitogen-activated protein kinase;
RANTES
- From:
Chinese Journal of Emergency Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of p38MAPK on the RANTES expression after unilateral ureteral obstruction.Methods Twenty-two rats were randomly assigned to shame operation group (normal group) and operation group after unilateral ureteral obstruction.Renal tissues were examined by light microscopy at 8 h、24 h and 72 h after operation,Immunhistochemistry was applied to measure the expression of RANTES,RT-PCR,Western blot was performed to determine mRNA,protein,respectively.Results Compared with shame opeartion group,the mRNA and protein levels of RANTES in renal interstitum of operation group remarkly increased (P＜0.05),and also stimulated the phosphorylation of p38MAPK.Conclusion The overexpression of RANTES in UUO rats by p38MAPK signal transduction pathway.
