Determination of baicalin and wogonin in Chantui Zhike Granules by HPLC
- VernacularTitle:HPLC测定蝉蜕止咳颗粒中黄芩苷和汉黄芩素含量
- Author:
Kai MA
;
Wenjie QIN
;
Lei BA
;
- Publication Type:Journal Article
- Keywords:
Chantui Zhike Granules;
HPLC;
baicalin;
wogonin
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the determination of baicalin and wogonin in Chantui Zhike Granules (Periostracum Cicadae, Radix Scutellariae, etc.) by HPLC. METHODS: The determination was carried out by HPLC. The mobile phase was acetonitrile 0.6% phosphoric acid(45∶55) and UV detection wavelength was set at 275nm. The flow rate was 1.0mL?min -1 . The column temperature was at 40 ?C . RESULTS: The contents of baicalin and wogonin in Chantui Zhike Granules could be determined by HPLC. There was a good linearity between the absorption peak area and the concentration in the range of 0.104?g～0.52?g for baicalin and in the range of 0.03?g～0.15?g for wogonin, respectively. The average recoveries were 100.69%( RSD =1.75%) and 98.8%( RSD =1.01%) respectively. CONCLUSION: The method is simple, accurate and can be used to determine the contents of baicalin and wogonin in Chantui Zhike Granules.
