PREGNANCY INCREASES CELL PROLIFERATION AND NEUROGENESIS IN THE MOUSE DENTATE GYRUS
- VernacularTitle:怀孕可增加小鼠海马齿状回的细胞增殖和神经发生
- Author:
Zhengang YANG
;
Lin YANG
;
Yingmao GAO
;
Jinhao SUN
;
Zhenhua LI
;
- Publication Type:Journal Article
- Keywords:
Pregnancy;
Neurogenesis;
Dentate gyrus;
Immunohistochemistry;
Mouse
- From:Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe cell proliferation and neurogenesis in the pregnant mouse dentate gyrus (DG) and the subventricular zone(SVZ). Methods Injection of the thymidine analog 5 bromo 2 deoxyuridine (BrdU)to determine the extent of cell proliferation combined with single or double immunohistochemistry staining with antibodies BrdU,TuJ1(class Ⅲ ? tubulin,neuron specific early differentiation marker)and GFAP. Results The number of BrdU positive cells in the pregnant mouse dentate gyrus was significantly more than that of unpregnant mouse dendate gyrus but not in the subventricular zone.In dentate gyrus,approximately 80% of these cells were neuronal characteristics (TuJ1 immunoreactive)and 3%～5% of these cells were astrocytic characteristics(GFAP immunoreactive).Conclusion\ These findings suggest that pregnancy significantly increases cell proliferation and neurogenesis in the adult mouse dentate gyrus and present the possibility that these new cells exert an important influence on hippocampal function
