Assay of berberine hydrochloride in Sanhuang Tablets by HPLC
- VernacularTitle:HPLC法测定三黄片中盐酸小檗碱的含量
- Author:
Xiaoyan LI
;
- Publication Type:Journal Article
- Keywords:
HPLC;
Sanhuang Tablets;
berberine hydrochloride
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the assay of berberine hydrochloride for Sanhuang Tablets (Radix et Rhizoma Rhei, Extractum Scutellariae and berberine hydrochloride). Methods:The HPLC with a KF C 18 column (250mm?4.6mm,10?m) was used. Acetonitrile-0.05 mol?L -1 triethylamine buffer solution (adjusted pH to 3.0 with conc. H 3PO 4) (35∶65) was used as mobile phase. The flow rate was 1.0mL?min -1 , and the detection wavelength was at 347nm. Results: The method has a good linear dependuce. The average recovery of loading sample was 98.21% with RSD 1.01%. ( n =9). Conclusion: The method is convenient for a good resolution and can be used for the quality control of Sanhuang Tablets.
