Determination and Analysis of Kaempferol in Sedum aizoon L.by HPLC-MS/MS
- VernacularTitle:养心草中山奈酚成分的HPLC-MS/MS分析及含量测定
- Author:
Suhua GUO
;
Zhucan LIN
;
Zhiwei ZHANG
;
- Publication Type:Journal Article
- Keywords:
Sedum aizoon L.;
kaempferol;
HPLC-MS/MS;
HPLC;
determination of content
- From:China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To discover and determine the content of kaempferol in Sedum aizoon L.for the first time.Methods: Waters HPLC-MS/MS,XTerra-MS C18 (5?m,2.1?150mm) and the mobile phase consisted of acetonitrile-water-formic(40∶60∶1) were applied to find kaempferol in Sedum aizoon L.;Daojin HPLC and the SHIM-PACK VP C18(250nm?4.6nm,10?m) column were used.The mobile phase consisted of methanol-0.4% phosphoric acid solutions(59:41) with the flow rate at 1.0ml/min and the UV detector wave-length were set at 370nm.Results: Compared with standard sample,the thing that kaempferol exists in Sedum aizoon L.was confirmed.The calibration curve was in good linearity over the range of 2.0-8.0?g,and regression equation was Y=40343X-11107(r=0.9998).The average recovery rate was 102.53%,with RSD =0.92%(n=6).Conclusion: The method is simple,accurate and reproducible so it can be used to determine and analyze the content of kaempferol in Sedum aizoon L.
