Effects of genistein and VEGFR on proliferation of ECV304 cells
- VernacularTitle:三羟异黄酮对脐静脉内皮细胞ECV304增殖影响与VEGFR的关系
- Author:
Xiaoping YU
;
Mantian MI
;
Jundong ZHU
;
- Publication Type:Journal Article
- Keywords:
genistein;
vascular endothelial growth factor receptor, protein tyrosine kinase
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of genistein in the growth inhibition in the tumor angiogenesis. Methods The effects of genistein and vascular endothelial growth factor receptor (VEGFR) on the proliferation of ECV304 cells were observed by cell growth curves and flow cytometry. The level of the phosphorylation of VEGFR and the activity of protein tyrosine kinase (PTK) in ECV304 cells were detected by immunoprecipitation and kinase activity analysis. Results Vascular endothelial growth factor (VEGF) alone could facilitate the proliferation of ECV304 cells, up regulate the phosphorylation and PTK activity of VEGFR, but genistein alone could inhibit the growth of ECV304 cells, stop the cell cycle mainly at the phase of G 2/M, induce apoptosis, and down regulate the phosphorylation and PTK activity of VEGFR ( P
