Study of Helicobacterpylori on p38MAPK signal transduction pathway activation in gastric epithelial cancer cells line MKN45
- VernacularTitle:幽门螺杆菌对人胃癌MKN45细胞p38MAPK信号转导通路激活作用的研究
- Author:
Qi LI
;
Zhong-Ze FAN
;
Jue SUN
;
Ning-Ning LIU
;
Li-Hong ZHOU
;
Qiong WU
;
Yan WANG
;
Hua SUI
;
Hong GAO
;
- Publication Type:Journal Article
- Keywords:
helicobacter pylori;
gastric cancer;
COX-2;
p38MAPK;
ATF-2;
signal transduction
- From:China Oncology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Cyclooxygenase-2 (Cyclooxygenase-2,COX-2) is an important rate-limiting enzyme that is responsible for transformation of arachidonic acid into prostaglandins(PGs).Although Helicobacter pylori(Hp) infection-induced gastric over-expression of COX-2 (COX-2) is an important factor of gastric cancer,the mechanism of COX-2 expression in gastric mucosa cells infected with Hp is still not clear.Our study was to reveal the effect of Hp on expression of COX-2(cyclooxygenase-2),the impact of p38MAPK signaling pathway in human gastric epithelial cancer cells line MKN45,and to investigate the potential mechanisms of expression of COX-2. Methods:The expression of COX-2 mRNA infection by standard Hp NCTC11637 in human gastric epithelial cancer cells line MKN45 was evaluated by real-time fluorogenic quantitative polymerase chain reaction (RFQ-PCR).The effect of infection by Hp on COX-2 expression,activation of p38MAPK and its downstream of the ATF-2 was assayed by Western blot.Results:The expression of COX-2 mRNA in MKN45 cells infected by Hp compared with control group,COX-2 mRNA had 3 fold,7.2 fold,5.1 fold,4.3 fold up-regulation after 3 hrs,6 hrs,9 hrs,12 hrs,respectively. COX-2 mRNA expression in each time group was significantly higher than that in the control group(P
