Effects of advanced glycation end-products on skin keratinocytes by NF-?B activation
- VernacularTitle:晚期糖基化终末产物对表皮角质形成细胞功能的影响及其机制
- Author:
Ming TIAN
;
Chun QING
;
Yi-Wen NIU
;
Jiao-Yun DONG
;
Shu-Wen JIN
;
Fei SONG
;
Lan-Nv HUA
;
Shu-liang LU
- Publication Type:Journal Article
- Keywords:
NF-KappaB;
Proliferation;
Emigration and immigration;
Apoptosis;
Advanced glycation end-products
- From:
Chinese Journal of Trauma
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of advanced glycation end-products(AGEPs)on the function of normal keratinocytes in vitro so as to explore the role of AGEPs in impaired wound healing. Methods Normal rat keratinocytes were incubated with different concentrations of AGEPs.After 48 hours of culturing,the cell proliferation rates were measured by MTT colorimetric determination.The cell cycle distributions and apoptosis were analyzed with flow cytometry,and the migration was investigated by 24-well fluorimetric cell migration assay kit by exposing to 100?g/ml AGEPs.Nuclear extracts from these cells were examined for binding of nucleotides containing NF-?B consensus by immunocytochemistry and EMSA in vitro.Results The proliferations of normal keratinocytes were significantly arrested and many cells were induced to early apoptosis compared with control ones(P＜0.05)by exposing to AGEPs for 48 hours. Meanwhile AGEPs also irritated keratinocytes migration compared with control ones(P＜0.05).Inhibiting the activation of NF-?B could partly recover the proliferation of keratinocytes,reverse apoptosis and attenu- ate migration.Conclusion AGEPs are correlated with the migration,proliferation and apoptosis of kera- tinocytes by NF-?B.
