Inhibition effects of epigallocatechin-3-gallste (EGCG) on cell cycle of hum an breast cancer cells MDA-MB-435 and its mechanism
- VernacularTitle:EGCG对人乳腺癌MDA-MB-435细胞株细胞增殖的抑制及机制
- Author:
He-Cheng LI
;
Jing-Song LU
;
Yi-Feng HOU
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
EGCG;
cell cycle;
breast cancer;
p21;
cutured tumor cells
- From:
China Oncology
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study the effect of EGCG(extracts fr om green tea) on cell cycle in human breast cancer cell line and its mechanism. Methods:Cell proliferation assay kit was used to produce the dose-response curve. Flow cytometric assay was used to evaluate the cell cycle changes on MDA-MB435 cells with and without EGCG. The expression of protein was detected by Western blot. Results:EGCG could inhibit the proliferation of the breast canc er cells MDA-MB-435, 40 ?g/ml EGCG induced G 0 /G 1 phase arrest and the entrance to S phase. Both mRNA and protein level of p21 waf1/cip1 were up regulated in MDA-MB435 cells when treated by 40?g/ml EGCG. Conclus ions:Green tea can inhibit the growth of human breast cancer cells, perhaps by means of p21 and therefore inhibiting the progression of the cell cycle.
