Vibramycin inhibits the expression of MMP-2 and the invasiveness of PC-3 cells in vitro
- VernacularTitle:强力霉素抑制PC-3细胞基质金属蛋白酶-2表达及侵袭的体外研究
- Author:
Xiaokang GAO
;
He WANG
;
Bo YANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Carcinoma;
Matrix metalloproteinase;
Vibramycin
- From:Chinese Journal of Urology
2000;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibition of vibramycin on the expression of matrix metalloproteinase 2 (MMP 2) and the invasiveness of androgen independent prostatic carcinoma cell line PC 3 in vitro. Methods Immunohistochemistry stain and transwell chamber were used to investigate the expression of MMP 2 in different concentration of vibramycin treated PC 3 cells and the invasive ability of different concentration of vibramycin treated PC 3 cell. Results The positive rate of MMP 2 in PC 3 cells was decreased at a concentration of 5 mg/L of vibramycin and decreased dramatically at the concentration of 10 mg/L.The cells moved through the membrane was (82.0?4.6)/field in the control group,while decreased to (26.1?3.6)?(7.2?2.2) and (3.3?0.7)/field in 5?10 and 20 mg/L vibramycin treated PC 3 cell respectively. Conclusions Vibramycin can inhibit the invasiveness and metastasis of PC 3 cells, the mechanism of which is related to the inhibition of MMP 2 in PC 3 cell.
