Gender specific profiles of human telomere shortening during aging
- VernacularTitle:人外周血白细胞端粒DNA长度变化与性别的关系
- Author:
Lu-Lu GE
;
Chao LIU
;
Xiao-Hui CHEN
;
Al ET
- Publication Type:Journal Article
- Keywords:
Forensic biological evidence;
Telomere restricted fragment(TRF);
Gender;
Aging
- From:
Chinese Journal of Forensic Medicine
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In human,both in vivo and in vitro,telomere shortening appears to be a major component of cell senescence and aging. However, gender specific human telomere shortening needs to be further characterized. Therefore our study is aimed at clarifying gender-dependent profiles of telomere shortening. Methods 123 peripheral blood samples were collected from healthy individuals of different ages. The mean telomeric restricted fragment (TRF) was measured using Southern Blotting with Dig-labeled probe. Results Distinguished dynamics profiles of telomere shortening were observed among different age groups. Conclusion The result indicates that there are gender specific dynamic profiles of telomere shortening. Therefore, the gender must be considered when an individual age is estimated by telomeric restricted fragment length assay.
