Effects of Kaempferol on the Skeletal Muscle of KKAy Mice via PI3K-AKT-GLUT4 Signaling
10.11842/wst.2016.07.009
- VernacularTitle:山柰酚对2型糖尿病小鼠骨骼肌PI3K-AKT-GLUT4信号通路的影响
- Author:
Zhuo ZHANG
;
Wen SUN
;
Tonghua LIU
;
Xuan GUO
;
Dan HOU
;
Guangyuan XU
;
Lili WU
;
Lingling QIN
;
Yi HOU
;
LU ZHANG
;
Chengfei ZHANG
- Keywords:
Kaempferol;
type 2 diabetes;
PI3K-AKT-GLUT4 signaling
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2016;18(7):1139-1143
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the effects of kaempferol on the skeletal muscle of KKAy mice via PI3K-AKT-GLUT4 signaling.Spontaneous type 2 diabetic KKAy mice were made up for the model group.After 6-week treatment of kaempferol by intragastric administration,key targets of PI3K-AKT-GLUT4 signaling were detected using biochemical and immunohistochemical technique,and western blot.It was found that the body weight,fast blood glucose and random blood glucose were decreased after kaempferol administration.ITT results show that blood glucose at 30 min after injecting insulin and the area under the curve drop were reduced in the kaempferol group,and so was the insulin resistance index in the kaempferol group.In addition,the mRNA expressions of PI3K,AKT and GLUT4 in the kaempferol group increased significantly,while the protein levels of AKT and GLUT4 were up-regulated by kaempferol administration.It was concluded that kaempferol can significantly regulate the blood glucose,body weight and insulin resistance in mice through activating the PI3K-AKT-GLUT4 signaling.
