Determination of Chlorogenic Acid in Honeysuckle Stem and Honeysuckle from Different Sources in Differ-ent Harvest Periods by HPLC
- VernacularTitle:HPLC法测定不同来源不同采收期忍冬藤和金银花中绿原酸含量
- Author:
Xia ZUO
;
Chunxia LIU
;
Jinxia YANG
;
Li CHEN
- Publication Type:Journal Article
- Keywords:
Different harvest periods;
Different sources;
Honeysuckle stem;
Honeysuckle;
Chlorogenic acid;
HPLC;
Determination
- From:
China Pharmacist
2015;(4):682-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of chlorogenic acid in honeysuckle stem and honeysuck-le from different sources in different harvest periods. Methods:A Waters C18 column(250 mm × 4. 6 mm,5 μm)was used. The mo-bile phase consisted of acetonitrile-0. 4% H3 PO4(10:90)with the flow rate of 1. 0 ml·min-1 . The detection wavelength was 327 nm and the column temperature was 30℃. An external standard method was established for the determination of chlorogenic acid in honey-suckle stem and honeysuckle in six different harvest periods from three sources. Results:There was a good linear relationship within the range of 0. 065-1. 300 μg for chlorogenic acid(r=0. 999 8). The content of chlorogenic acid in honeysuckle was the highest in Sep-tember and October. The content of chlorogenic acid in Lonicera acuminata was the highest among the honeysuckle stem from three dif-ferent sources. Conclusion:The content of chlorogenic acid in honeysuckle has a certain relationship with the harvest time,which can provide theoretical basis for the choice of harvest time for honeysuckle stem.
