Extraction Process Optimization of Total Flavonoids in Gynostemma Pentaphyllum by Response Surface A-nalysis
- VernacularTitle:响应面分析法优化绞股蓝总黄酮的提取工艺
- Author:
Yan SONG
;
Zhanyi WANG
;
Yuhai WANG
;
Dandan ZHENG
;
Jinglong WANG
- Publication Type:Journal Article
- Keywords:
Gynostemma pentaphyllum;
Flavonoids;
Extraction technology;
Response surface analysis
- From:
China Pharmacist
2015;(5):714-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction process of total flavonoids in Gynostemma pentaphyllum by the method of response surface analysis ( RSM) . Methods: Based on the choice of factors and levels according to the results of single factor test, Box-Be-hnken response surface experiment was designed with the extraction rate of total flavonoids as the index to obtaln the best operation con-ditions and two equations of the response surface model. Results: The optimal extraction conditions of total flavonoids in Gynostemma pentaphyllum were as follows:the volume fraction of ethanol was 71%, the ratio of solid to liquid was 1∶14, and the ultrasonic time was 32 min. Under the conditions, the model predictive value of extraction rate of total flavonoids in Gynostemma pentaphyllum was 4. 676%, and the extraction rate in the verification test was 4. 641%. Conclusion:The fit of the regression model is good, and the ex-traction technology is feasible and reliable.
