MiR-107 target NID2 regulating invasion and proliferation of lung cancer by JAK/STAT signaling pathway
10.3969/j.issn.1000-484X.2017.11.014
- VernacularTitle:miR-107靶向NID2通过Notch信号通路调控肺癌细胞的增殖侵袭
- Author:
Xia WU
1
;
Jun HE
;
Jun Wen WANG
Author Information
1. 西南医科大学附属第一医院呼吸内科
- Keywords:
miR-107;
Lung cancer;
Notch;
A549;
Invasion;
Migration
- From:
Chinese Journal of Immunology
2017;33(11):1662-1667
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-107 targeting NID2 on Notch signaling pathway on invasion and proliferation of lung cancer.Methods: The expression of NID2 in lung cancer and normal tissues was detected by immunohistochemistry.The expression of miR-107 in lung cancer cell line was detected by PCR.The effect of miR-107 on the expression of NID2 was examined by the dual luciferase gene system.Effect of miR-26 on the proliferation ability of H1650 were detected by tumor in ball assays.Transwell invasion assay was used to detect the ability of invasion in the lung cancer cell line A549 after overexpression the miR-107.Scratch test was used to detect the ability of migration in the lung cancer cell line A549 after overex-pression the miR-107.Western blot was used to detect the protein expression of Notch signal pathway after overexpression miR-107.Results:The expression of NID2 in lung cancer tissues was significantly higher than that in normal lung tissues.The expression of miR-107 in lung cancer tissues was significantly lower than that in normal lung tissues.The dual luciferase reporter gene system showed that miR-107 could directly regulate the transcriptional activity of NID2.After miR-107 overexpression,the proliferation,invasion and migration ability of lung cancer cell A549 were significantly decreased.The expression of miR-107 protein could down-regulate the expression of Notch1,hes-1 and presenilin1 protein.Conclusion: miR-107 targets the expression of NID2 and regulates the invasion and proliferation of lung cancer cells by Notch pathway.
