Protective Effect of Rapamycin against Mitochondrial Injury in an Aging Parkinson's Disease Mouse Model
10.12007/j.issn.0258-4646.2017.09.004
- VernacularTitle:雷帕霉素对老龄帕金森病模型小鼠线粒体损伤的改善作用
- Author:
Zhongxia ZHANG
1
;
Xiaowei MA
;
Yanyong WANG
;
Huiqing QIU
;
Mingwei WANG
Author Information
1. 河北医科大学第一医院神经内科
- Keywords:
rapamycin;
aging;
Parkinson's disease;
mitochondria
- From:Journal of China Medical University
2017;46(9):783-786
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of rapamycin (RAPA) on mitochondrial injury in a mouse model of aging Parkinson's disease (PD).Methods Forty senescence-accelerated prone mice 8 (SAMP8) (12-month old) were randomly divided into 5 groups:blank group,model group,and RAPA low-,middle-,and high-dose groups.Mice in the model group and three RAPA groups were administered a subcutaneous injection of MPTP to generate the PD model.RAPA at 1,2,and 4 mg· kg 1· d 1 was administered from 7 days before,5 days during,and 7 days after the PD model preparation to the RAPA groups;an equal volume of sterile saline was administered to the other two groups.After the administration,behavioral test scores,dopamine levels,transmembrane potential of mitochondria,and activity of mitochondrial complex Ⅰ in the 5 groups were evaluated.Results Behavioral scores,dopamine levels,transmembrane potential,mitochondrial complex Ⅰ activity of mice in the model group were significantly decreased compared with the blank group (P ＜ 0.05 respectively).All indexes in the RAPA groups were significantly improved compared to the model group (P ＜ 0.05 respectively).There was no significant difference among the three RAPA groups.Conclusion RAPA has a protective effect on aging PD model mice,and its mechanism may be related to the protection against mitochondrial damage.
