Effect of grape seed proanthocyanidins on phenotype marker protein of renal cells in db/db mice
10.13315/j.cnki.cjcep.2017.08.007
- VernacularTitle:葡萄籽原花青素对糖尿病db/db小鼠肾组织细胞表型转化的影响
- Author:
ying Jin WEI
1
;
hong Yong SHI
;
zhuo Yong REN
;
xia Yun DU
;
yang Chun DU
;
jun Hui DUAN
;
jiang Hai WU
Author Information
1. 河北医科大学病理学教研室
- Keywords:
diabetic nephropathy;
epithelial to mesenchymal transition;
grape seed proanthocyanidin extract;
α-SMA;
E-cadherin
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(8):858-862
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the effect of grape seed proanthocyanidins on the phenotype-transforming marker protein expression of db/db renal cells in mice model of type 2 diabetes,and to explore the protective mechanism of grape seed extract on diabetic renal injury in db/db mice.Methods Male db/db diabetic mice were randomly divided into two groups:diabetic group (db/db group) and diabetic + grape seed proanthocyanidin extract group (db/db + GSPE).The same week-old male db/m mice was used as normal controls (db/m) and grape seed proanthocyanidin extract gavage treatment group (db/m +grape seed proanthocyanidin extract group,db/m + GSPE).The mice of db/db + GSPE group and db/m + GSPE group were administered daily with grape seed proanthocyanidin extract (5mg/kg) by gavage.Results Renal tissues of db/db diabetic mice showed increased expression of α-SMA,p-p38MAPK,pERK1/2 and 8-OHdG level,and down-regulation in E-cadherin expression compared with db/m group (P ＜ 0.05).However,the alternations of α-SMA,p-p38,p-ERK1/2,E-cadherin protein levels,and 8-OHdG level,in db/db group were reversed by addition of grape seed proanthocyanidin extract (P ＜ 0.05).Conclusion Grape seed proanthocyanidin extract inhibits the epithelial to mesenchymal transition (EMT) associated protein,by decreasing ROS production,and activating p38 MAPK and ERK1/2.These findings suggest that grape seed proanthocyanidin extract provides a treatment option for diabetic nephropathy.
