Research on the protective effect of glycyrrhizin against renal ischemia-reperfusion injury in mice and its mechanisms
10.3760/cma.j.issn.0254-1785.2017.09.011
- VernacularTitle:甘草素减轻小鼠肾脏缺血再灌注损伤的作用及其机制研究
- Author:
Shaojun YE
1
;
Zibiao ZHONG
;
Zhongzhong LIU
;
Qianchao HU
;
Xiaoli FAN
;
Kai JING
;
Min ZHOU
;
Yan XIONG
;
Yanfeng WANG
;
Qifa YE
Author Information
1. 430071,武汉大学中南医院武汉大学肝胆疾病研究院武汉大学移植医学中心移植医学技术湖北省重点实验室
- Keywords:
Glycyrrhizin;
Ischemia;
Reperfusion injury;
Kidney function;
Mechanisms
- From:Chinese Journal of Organ Transplantation
2017;38(9):564-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of glycyrrhizin against renal ischemiareperfusion injury in mice and its mechanisms.Methods Male C57BL/6 mice were divided into three groups of six.Bilateral flank incisions were made,the right kidney was removed and the left kidney was subjected to ischemia using a microvascular clamp,which was removed after 30 min.In the shamoperated group,the mice underwent anesthesia,bilateral flank incisions and a right nephrectomy.In the glycyrrhizin-treated group,the mice were injected with 60 mg/ kg glycyrrhizin 1 h prior to ischemia.In the saline-treated group,the mice were administered with 60 mg/ kg saline.The mice were sacrificed 6 h after reperfusion and the blood and kidney samples were immediately collected for kidney function,inflammatory response and signal pathway test.Results As compared with those in the saline-treated group,the mice in glycyrrhizin0-treated group exhibited notably decreased serum levels of creatine and blood urea nitrogen at 6 h following reperfusion (P＜0.01),the SOA level was significantly reduced (P＜0.01) and the SOD activity was increased.The activity of MPO (P＜0.01)in the glycyrrhizin-treated group was significantly reduced as compared with the saline-treated group,also the serum level of pro-inflammatory TNF-α (P＜0.05),IFN-γ (P＜0.05),IL-1β (P＜0.01) and IL-6 (P＜0.01).Furthermore,the phosphorylated-p38 protein level in the glycyrrhizin-treated group was notably as reduced compared with that in the saline-treated group.Conclusion Pretreatment with glycyrrhizin attenuates renal ischemia-reperfusion injury via inhibition of tissue inflammation by downregulating p38 mitogen-activated protein kinase signaling.
