Role of RNA-binding protein HuR in human adipocyte differentiation
10.3760/cma.j.issn.1000-6699.2017.10.010
- VernacularTitle:RNA结合蛋白人抗原R在人脂肪细胞分化中的作用
- Author:
Dan ZHANG
1
;
Xiaodi LIANG
;
Nuerbiye NUERMAIMAITI
;
Ling LIU
;
Xuanyu MENG
;
Jie LIU
;
Yi JIAO
;
Jianfei LU
;
Yaqun GUAN
Author Information
1. 新疆医科大学基础医学院生物化学与分子生物学教研室
- Keywords:
Adipocyte differentiation;
RNA binding protein;
Human antigen R;
Adipocyte fatty acid binding protein;
Fatty acid synthase;
Lipoprotein lipase
- From:Chinese Journal of Endocrinology and Metabolism
2017;33(10):854-860
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of RNA-binding protein human antigen R(HuR), fatty acid binding protein type 4(FABP4),fatty acid synthetase(FASN),and lipoprotein lipase(LPL)during the differentiation of human adipocytes, and to explore their possible roles. Methods Human adipose-derived mesenchymal stem cells were induced by adipogenic differentiation,and the adipogenesis of cells was observed by oil red O staining. The expressions of HuR,FABP4,FASN,and LPL mRNA and protein were detected by real-time PCR and Western blotting. After HuR was silenced by siRNA, the change of adipogenesis for human adipose-derived mesenchymal stem cells was observed and the expressions of adipogenic genes were detected. Results The expressions of HuR,FABP4,FASN,and LPL mRNA and protein were significantly increased after human adipose-derived mesenchymal stem cells were induced to differentiate into adipocytes(all P<0.01). After HuR expression was down-regulated by siRNA,the adipogenic level of human adipose-derived mesenchymal stem cells was reduced,with decreased protein levels of FABP4,FASN,and LPL(all P<0.05),which were without changes for their mRNA levels. Conclusion HuR promotes the differentiation of human adipocytes mainly via regulating the changes of FABP4,FASN,and LPL protein levels.
