Effects of zedoary turmeric oil on cell proliferation and apoptosis and Caspase-3, Bax, Bcl-2 protein expression in rectal carcinoma cell line SW1463
10.7501/j.issn.1674-6376.2017.07.003
- VernacularTitle:莪术油对直肠癌SW1463细胞株增殖、凋亡及Caspase-3、Bax、Bcl-2蛋白表达的影响
- Author:
xun Bin LIAO
1
;
Chao TANG
;
song Nian PAN
;
fang Hui ZHAO
;
Jun LUO
Author Information
1. 黔西南州人民医院
- Keywords:
rectal cancer cells;
zedoary turmeric oil;
cell proliferation;
caspase-3;
Bax;
Bcl-2;
cell apoptosis
- From:Drug Evaluation Research
2017;40(7):897-903
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of zedoary turmeric oil on proliferation and apoptosis of SW1463 cell line and the expression of Caspase-3,Bax and Bcl-2.Methods Volatile oil from Curcumae Rhizoma in Guizhou was extract by steam distillation,which was used to intervene SW1463 cells for 24,48 and 72 h at concentration of 40,80,120,160,200,240 and 280 mg/mL.MTT method was used to detect the inhibitory rate of zedoary turmeric oil on SW1463 cell proliferation.Effects of different concentrations of zedoary oil on apoptosis of SW1463 cells were observed by Giemsa staining.Western blotting was used to detect Capase-3,Bax and Bcl-2 protein expression.Results Zedoary turmeric oil inhibited the proliferation of SW1463 cells and showed a time dose correlation,and half maximal inhibitory concentration (IC50) of 24,48 and 72 h was 144.33,134.11 and 120.04 mg/L,respectively.Giemsa staining showed obvious morphological characteristics of apoptotic cells.Western blotting results showed that compared with control group,the expression of Caspase-3 and Bax in cells treated with zedoary turmeric oil for 24 h were significantly up-regulated (P ＜ 0.05),and the expression of Bcl-2 protein was significantly down-regulated (P ＜ 0.05).Conclusion Zedoary turmeric oil can obviously inhibit the proliferation of SW1463 cells and induce apoptosis,which may be related to the up-regulation of Caspase-3 and Bax protein expression and down-regulation of Bcl-2 protein expression.
