Antitumor activity and acute toxicity of total saponins from Paris forrestii
10.7501/j.issn.1674-6376.2017.07.004
- VernacularTitle:长柱重楼总皂苷的抗肿瘤活性及急性毒性作用研究
- Author:
mei Xue CHEN
1
;
zhi Huan YANG
;
Min SHI
;
rong Rong YANG
;
jie Ying CHEN
;
hu Yue WANG
;
ping Yi ZHOU
Author Information
1. 昆明医科大学药学院暨云南省天然药物药理重点实验室
- Keywords:
Paris forrestii (Takht) H.Li;
total saponins;
antitumor activity;
mouse transferred hepatocellular carcinoma;
acute toxicity;
organ coefficient
- From:Drug Evaluation Research
2017;40(7):904-910
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the antitumor activity of total saponins form Parisforrestii (PCT3) in vitro and in vivo and its acute toxicity by disposable ig administration.Methods The inhibitory effect of PCT3 on proliferation of human colorectal cancer cells (HCT-116) and human gastric cancer cells (SGC-7901) was detected by modified MTT assay,and the half inhibition concentration (IC50) was calculated.Acute toxicity test of PCT3 at doses of 1 646,2 352,3 360 and 4 800 mg/kg was performed by ig administration in mice.Mouse model of hepatocellular carcinoma (H22) was established with sc injection,and the mice were respectively ip given cisplatin 2 mg/kg (positive control),normal saline (negative control),ig given 0.5％ CMC-Na (solvent control),30,90 and 270 mg/kg PCT3 continuously for 9 d,and the inhibitory rate of tumor,body weight,liver,spleen,kidney and thymus coefficients were detected.Results PCT3 had significant inhibitory effect on the proliferation of HCT-116 and SGC-7901 cells,with IC50 values of 7.6 and 5.9 μg/mL,respectively.PCT3 induced animal diarrhea and activity inhibition in mice,the half lethal dose (LD50) was 1985.5 mg/kg.Compared with solvent control group,PCT3 had no significant effect on body weight of mice;270 mg/kg PCT3 had a significant inhibitory effect on H22 tumor mass (P ＜ 0.05),the inhibitory rate was 26.8％;There was no significant effect on the organ coefficient;compared with negative control group,cisplatin significantly inhibited the tumor growth and body weight (P ＜ 0.01),the inhibitory rates were 81.4％ and 37.4％ respectively;The liver,spleen and thymus coefficients were also significantly inhibited (P ＜ 0.05,0.01).Conclusion The tumor inhibitory rate of cisplatin is significantly higher than that of PCT3,but it also significantly inhibits mice body weight and liver,spleen,thymus coefficients.PCT3 shows obvious antitumor activity in vitro and in vivo,and the toxicity is not remarkable.It could be a potential antitumor agent in the future.
