Protection of Batroxobin Injection on super acute stage of thromboembolic cerebral stroke in rats
10.7501/j.issn.1674-6376.2017.07.005
- VernacularTitle:巴曲酶注射液对大鼠血栓栓塞性脑卒中急性超早期损伤的保护作用
- Author:
hua Chun HAO
1
;
Rui ZHANG
;
yong Shuang SUN
;
ting Wei WANG
;
you Zhuan ZHAO
;
da Li TANG
Author Information
1. 天津药物研究院新药评价有限公司
- Keywords:
Batroxobin Injection;
rat;
thromboembolic cerebral ischemia;
magnetic resonance imaging;
TTC staining
- From:Drug Evaluation Research
2017;40(7):911-916
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Batroxobin Injection on thromboembolic cerebral stroke by magnetic resonance imaging (MRI) and TTC staining.Methods Rat model ofthromboembolic stroke was prepared after the left middle cerebral artery was occluded by autologous blood clots,and 32 rats with successful operation were divided into four groups according to the degree of neurological deficit:model group,Batroxobin Injection low and high dose (0.3,1.0 BU/kg) group,and rt-PA (9 mg/kg) group,with eight rats in each group,and other eight rats in Sham group.Rats were administered 1 h after modeling by tail iv method.At 6 h after administration,neurological deficit score and MRIincluding SE-T2WI and DWI sequence scanning were measured.At 24 h after administration,the brain was cut for TTC staining to measure the infarct area,and blood FIB was measured.Results Compared with model group,Batroxobin Injection 0.3 BU/kg treatment for 24 h (P ＜ 0.05),1 BU/kg treatment for 6 and 24 h (P ＜ 0.05,0.01) could significntly improve the neurological function scores of rats.MRIresults showed that Batroxobin Injection at dose of 0.3 and 1 BU/kg significantly reduced the lesion range (P ＜ 0.05 and 0.01).Results of TTC stain showed that Batroxobin Injection at dose of 0.3 and 1 BU/kg significantly reduced the infarct size (P ＜ 0.05).Batroxobin Injection at doses of 0.3 and 1 BU/kg can significantly lower plasma FIB concentration (P ＜ 0.05,0.01,0.001) 6 and 24 h after administration.Conclusion Batroxobin Injection can improve the damaged neural function,reduce scope of lesions,decrease plasma fibrinogen,with protective effects for cerebral ischemia in rats.
