The effect of high-fat diet-induced obesity on oxidative stress and klotho methylation in lung tissue of C57/BL6 mice
10.3969/j.issn.1006-5725.2017.18.010
- VernacularTitle:高脂饮食对C57/BL6小鼠肺组织氧化应激和klotho启动子甲基化的影响
- Author:
Lu YU
1
;
Yuxia WANG
;
Caiyu JIANG
;
Jiang HUANG
Author Information
1. 四川省医学科学院·四川省人民医院呼吸内科
- Keywords:
obesity;
lung;
oxidative stress;
klotho;
methylation
- From:
The Journal of Practical Medicine
2017;33(18):3017-3020
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of high-fat diet-induced obesity on oxidative stress and klotho promoter methylation in lung tissue of C57/BL6 mice. Methods Mice in the control group were feed with the normal diet,and mice in the obesity group were feed with high-fat diet. The lung tissue level of uperoxide dis-mutase(SOD)and malondialdehyde(MDA)were determined by using mice enzyme-linked immunosorbent assay (ELISA)kit. The klotho mRNA and protein expression was determined by qPCR and Western-blot ,respectively. The Klotho gene methylation status was determined by methylation specific PCR(MS-PCR). Results Compared with the control group,mice in the obesity group had high level of oxidative stress in lung tissue. Meanwhile,mice in the experimental group had lower levels of klotho mRNA and protein expression than those in the control group. The high-fat diet increased the degree of Klotho gene methylation. Conclusion High-fat diet could lead injure in lung tissue in C57/BL6 mice,klorho promoter methylation may play an important role involved in the process.
