Therapeutic effects of oleanolic acid nanoparticles combined with heparin sodium nanoparticles on liver cancer in vivo and in vitro
10.7652/jdyxb201706012
- VernacularTitle:齐墩果酸纳米粒与肝素钠纳米粒联合应用治疗肝癌的体内外研究
- Author:
han Xiao JIN
1
;
han Ying LIU
;
qian Rong XU
;
Yu CHEN
;
Xu BAO
;
bin Jian ZHANG
;
Meng GAO
;
Yan TIAN
Author Information
1. 大连医科大学
- Keywords:
OPTN;
HPTN;
use in combination;
liver cancer;
apoptosis;
synergistic effect
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(6):844-850
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects of oleanolic acid-loaded polylactic-co-glycolic acid-D-α-tocopheryl polyethylene glycol 1000 succinate (PLGA-TPGS ) nanoparticles (OPTN ) combined with heparin sodium-loaded PLGA-TPGS nanoparticles (HPTN)on liver cancer,and explore whether OPTN combined with HPTN has a synergistic effect by comparing the results of single medication.Methods Coumarin 6 and eosin were used as fluorescent probes to examine the cellular uptake by human liver cancer HepG2 cell and murine ascitic hepatocarcinoma cell strain with high metastasis potential in the lymphatic system (HCa-F).The in vitro cytotoxic combination effect and apoptosis of HepG2 cells induced by OPTN combined with HPTN were also determined using WST-1 assay and Annexin V-FITC staining. The antitumor effect in vivo was determined by tumor growth inhibition rate and hematoxylin & eosin staining (H & E)method.Results Both OPTN with green fluorescence and HPTN with red fluorescence were found in HepG2 cells and HCa-F cells,suggesting that they had been internalized.The cell cytotoxicity test and Annexin V-FITC staining results showed that OPTN combined with HPTN had a synergistic effect.The therapeutic effect in vivo showed that OPTN combined with HPTN effectively inhibited tumor growth better than the drug alone.Conclusion OPTN combined with HPTN has a synergistic effect in more effectively inhibiting liver cancer better than single medication.
