Effect of Ginkgo biloba extract EGb761 on apoptosis of K-ras mutational human colon cancer cells DLD1 and its mechanism

VernacularTitle:银杏叶提取物EGb761诱导K-ras突变型人结肠癌DLD1细胞凋亡及其机制
Author: Guangyu JI ¹ ; Yao CHENG ; Qizhao MA ; Yanjiang ZHENG ; Shiping LIAO ; Yufang WANG ; Sutang GUO
Author Information

1. 山西医科大学基础医学院
Keywords: Colonic neoplasms; Ginkgo biloba extract; Mutation; Apoptosis; NF-κB signaling pathway
From: Cancer Research and Clinic 2017;29(9):584-588,597
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of Ginkgo biloba extract (EGb761) on apoptosis of K-ras mutational human colon cancer cells DLD1(DLD1/G13D)and its mechanism. Methods Human colon cancer cell lines DLD1/G13D and DLD1 with K-ras wild type(DLD1/WT)were cultured in vitro,the cell proliferation and apoptosis after 24 h of EGb761 were measured. Proteins involved in related signal pathway were detected by Western blot or ELISA. Results EGb761 reduced cell proliferation and induced cell apoptosis in a concentration-dependent manner in DLD1/WT and DLD1/G13D cells. EGb761 downregulated the expression of RIP1, impaired the phosphorylation of IκB and decreased the level of NF-κB in DLD1/WT and DLD1/G13D cells[DLD1/G13D: (24±4)%, DLD1/WT: (29±9)%(P<0.05). Conclusion EGb761 restrains the proliferation and induces the apoptosis of DLD1/WT and DLD1/G13D cells. The mechanism may be related to the degradation of RIP-1 and inhibition of activation of NF-κB signaling pathway.