Observation of the articular cartilage of osteoarthritis in SIRT1 gene knock-out mice through single staining and compound staining
10.3969.j.issn.1671-7856.2017.10.007
- VernacularTitle:单染与复染在观察SIRT1基因敲除小鼠骨关节炎切片中的差异比较
- Author:
yan Hong YU
1
;
dong Zhan YANG
;
Qi YANG
;
Fei YU
Author Information
1. 滨州市滨城区市立医院
- Keywords:
SIRT1 gene;
Gene knockout;
Mouse model;
Osteoarthritis;
Single staining;
Compound staining
- From:Chinese Journal of Comparative Medicine
2017;27(10):34-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish osteoarthritis model of the knee joint in mice on the basis of knocking out SIRT1 gene and to observe the differences in the morphology of the cartilage tissue using single staining and compound staining. Methods The knee joint specimens were divided into two groups: SIRT1 -/ - control group ( group A, n=6 ) and SIRT1 -/ - osteoarthritis model group ( group B, n=6 ) . The knee anterior cruciate ligament was traversed, and the ipsilateral medial meniscus was cut to establish an osteoarthritis model of knee joint. HE staining, safranin O-fast green staining, safranin O-alcian blue staining, safranin O staining, fast green staining, alcian blue staining were used to observe the morphological changes in the articular cartilage of the knee. Results Safranin O-fast green staining and safranin O-alcian blue staining showed better results in observation of the morphology of chondrocytes, the structure of cartilage layers, the presence of type II collagen, tide line and the changes of subchondral bone. While the safranin O staining and alcian blue staining had certain advantages in the observation of the defects of cartilage tissue. Conclusions Compared with the single staining, the compound staining used in this study have obvious advantages in obtaining useful information of the cartilage structure in the observation of morphology of cartilage tissues in SIRT1 gene knock-out mice.
