Research of berberine to proliferation and apoptotic mechanism of human ovarian cancer cell line SKOV3
10.3969/j.issn.1000-484X.2017.10.012
- VernacularTitle:小檗碱对人卵巢癌细胞SKOV3增殖及凋亡机制的研究
- Author:
Wan WANG
1
;
Fan ZHANG
;
Hui YUAN
;
Jing Wen YANG
Author Information
1. 江汉大学附属医院(武汉市第六医院)妇产科
- Keywords:
Berberine;
Methylation;
Mismatch repair gene;
Ovarian cancer SKOV3 cells
- From:
Chinese Journal of Immunology
2017;33(10):1498-1501,1508
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of berberine on the proliferation and apoptosis of human ovarian cancer cell (SKOV3). Methods:Cell proliferation was detected by MTT method. The cell apoptosis was detected by FCM Annexin V/PI double staining and transmission electron microscopy. The methylation status of hMLH1 gene promoter CpG island was analyzed by methylation specific PCR. The expression of Bcl-2, Bax, Survivin and hMLH1 gene mRNA were detected by real-time fluorescent quantitative RT-PCR. Results:The berberine could significantly inhibit the proliferation of ovarian cancer SKOV3 cells(P<0. 05) in dose-and time-de-pendent manner. When combined with cisplatin, berberine showed synergistic anticancer effects. Berberine could induce SKOV3 cells apoptosis significantly, it might lower the expression of Bcl-2 and Survivin gene and enhance the expression of Bax gene. In addition, berberine could restore the hMLH1 promoter methylation status and increase the expression of hMLH1 mRNA. Conclusion:Berberine can inhibit the proliferation of ovarian cancer cells and induce apoptosis, which show that the synergistic enhancement anticancer effects with cisplatin.
