Effects of advanced glycation end products on oxidative stress in endothelial cells
- VernacularTitle:糖化终末产物对内皮细胞氧化应激的影响
- Author:
qin-min, GE
;
fan, BIAN
;
qin, XU
;
qing, SU
- Publication Type:Journal Article
- Keywords:
advanced glycation end products;
endothelium;
oxidative stress;
reactive oxygen species
- From:Journal of Shanghai Jiaotong University(Medical Science)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of advanced glycation end products(AGEs) on the oxidative stress in porcine vascular endothelial cells(PIECs). Methods After being intervened by AGEs for some time,cell viability was detected by MTT.2′,7′-dichlorofluorescein diacetate(DCFH-DA) was used as a reactive oxygen species(ROS) capture agent.The fluorescent intensity of 2′,7′-dichlorofluorescein(DCF),which was the product of cellular oxidation of DCFH-DA,was detected by flow cytometry,and the level of ROS was thus measured. Results Viability of PIECs was inhibited by AGEs in a dose-and time-dependent fashion(P
