Protective Effects of Astragalus on Human Renal Tubular Cells Injury Induced by Postasphyxial-Serum from Neonate
- VernacularTitle:黄芪注射液对新生儿窒息后血清诱导人近曲肾小管上皮细胞损伤的保护作用
- Author:
tao, XIONG
;
wen-bin, DONG
;
ming-yong, WANG
;
cun-liang, DENG
- Publication Type:Journal Article
- Keywords:
astragalus;
asphyxia;
renal injury;
infant,newborn
- From:Journal of Applied Clinical Pediatrics
2006;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effects of astragalus on injury of renal tubular cells(HK-2) induced by postasphyxial-serum from neonate.Methods Human renal proximal tubular cell line HK-2 cells were taken as subjects.The experiment was designed as:control group,model group,astragalus pretreatment group.The astragalus pretreatment group had 5 subgroups,which were pretreated for 24 hours.The serum of neonates who suffured asphyxia for 1 day,which concentration were 200 mL/L(volume fraction),were applied as attacking factor.The injury of morphology were observed under inverted microscope.The cell viability was measured by methyl thiazolyl tetrazolium methods.The leakage rate of lactate dehydrogenase(LDH) was determined by biochemical methods.Results Compared with control group,cell morphous changed,leakage rate of LDH elvated and cell survival rate decreased in model group,the variances were obviously significant(Pa
