Inhibitory Effect of Silybin-Phosphatidylcholine Compound on the Activation of Nuclear Factor-?B and Phosphorylation of Inhibitors of Nuclear Factor-?B ? in Lipopolysaccharide Induced by Mouse Phagocyte
- VernacularTitle:水飞蓟宾-磷脂酰胆碱复合物对脂多糖诱导小鼠巨噬细胞核因子-?B活化及核因子-?B抑制蛋白?磷酸化的影响
- Author:
yun-qi, GUO
;
yun-lan, GUO
;
yun, LV
;
zheng-yue, CHEN
- Publication Type:Journal Article
- Keywords:
silybinin;
inflammation;
lipopolysaccharide;
nuclear factor-?B;
inhibitors of nuclear factor-?B?
- From:Journal of Applied Clinical Pediatrics
2006;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of silybin-phosphatidylcholine compound (SPC )on lipopolysaccharide (LPS)-induced activation of nuclear factor-?B (NF-?B) and phosphorylation and degradation of inhibitors of NF-?B?(I?B?).Methods Phagocyte were collected in abdominal cavity of Kunming mousse aged 6 to 8 weeks,cultured phagocyte(2?105 mL-1) were divided into control, LPS and SPC groups randomly, phagocyte in control group were added into the same volume of 9 g/L sodium chloride.Phagocyte in LPS group were added into a single bolus of LPS(10 ?g/mL LPS) for 24 hours, phagocyte in SPC groups were preincubated with different concentration of SPC for 2 hours followed by a 24 hours incubation with 10 ?g/mL LPS. Immunocytochemistry were used to measure the contents of NF-?B, phosphorylated I?B? in phagocyte.Results The content of NF-?B p65 located in the nuclear in control group was little. The content of NF-?B p65 located in the nuclear in LPS group markly higher than that in control group(P
