Investigation of ocular structure in pigmented rabbit and C57BL/6 mouse using spectral-domain optical coherence tomography
10.3760/cma.j.issn.2095-0160.2013.11.012
- VernacularTitle:频域光学相干断层扫描对C57BL/6小鼠及色素兔眼前后节结构特点的活体观察
- Author:
Xiao-gang, WANG
;
Shuai, WANG
;
Shan-shan, DU
;
Qiang, WU
- Publication Type:Journal Article
- Keywords:
Tomography/optical coherence tomography/spectral-domain;
Mouse/C57BL/6;
Pigmented rabbit;
Cornea;
Retina
- From:Chinese Journal of Experimental Ophthalmology
2013;32(11):1055-1059
- CountryChina
- Language:Chinese
-
Abstract:
Background Spectral-domain optical coherence tomography (SD-OCT) can quantitatively analyze some ocular parameters in vivo.Although human ocular parameters have been obtained by SD-OCT,few studies were performed in animal experiment.Objective This study was to investigate the anterior and posterior segment parameters of C57BL/6 mice and pigmented rabbits using SD-OCT in vivo.Methods Some anterior and posterior segment ocular parameters,including the central corneal thickness (CCT),anterior chamber depth (ACD),white-to-white (WTW),optic nerve head (ONH) depth/width and retinal thickness,were measured in 8 eyes of 4 health SPF C57BL/6 mice and 12 eyes of 6 health SPF pigmented rabbits using SD-OCT.Results For C57BL/6 mice,Cornea,iris,lens in pupil area were clearly exhibited by SD-OCT.Mean CCT,ACD and WTW were (96±9)μm,(460±8) μm and (2.86 ± 0.41) mm pre-mydriasis,respectively,the corresponding values of post-mydriasis were (96±8) μm,(356±20)μm and (2.87±0.62)mm.There were no statistical differences of CCT and WTW between pre-and post-mydriasis (t =0.478,P =0.647 ; t =-0.737,P =0.485).ACD of post-mydriasis was significantly shallower than that of baseline (t =-13.022,P＜0.001).For the pigmented rabbits,the thickness of corneal thinnest point,retinal thickness,ONH depth and width were (370 ± 10) μm,(175 ± 4) μm,(1.35 ± 0.51) mm and (4.52±0.82) mm,respectively.Conclusions As a non-contact and non-invasive technology,SD-OCT can provide not only high resolution cross-sectional ocular images,but also high precise quantitative parameters for both C57BL/6 mouse and pigmented rabbit in vivo.
