Optimization of bioethanol production process using oil palm frond juice as substrate
- Author:
Maya Emira Mad Saad
;
Abdul Halim Farah Amani
;
Lee Chee Keong
- Publication Type:Journal Article
- Keywords:
Oil palm frond juice;
Saccharomyces cerevisiae HC10;
bioethanol;
response surface methodology;
optimization
- From:Malaysian Journal of Microbiology
2016;12(4):308-314
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Bioethanol is an environmental friendly energy source with a lot of great prospective and become an alternative to
fossil fuels .Oil palm frond juice (OPFJ) is a potential sources of sugars for bioethanol production. The present study
aimed to optimize bioethanol production.
Methodology and results: Bioethanol fermentation was carried out by Saccharomyces cerevisiae HC10 using OPFJ as
substrate in bioreactor with 1.5 L working volume. Growth profile was performed for 42 h with sampling every 3 h
interval. Effect of agitation speed (rpm) and volume of OPFJ were screened to select significant factor for high production
of bioethanol. Agitation speed at 175 rpm and volume of oil OPFJ; 40% gave 5.25 g/L and 4.52 g/L of ethanol and
biomass concentration, respectively. These parameters were further investigated via central composite design (CCD) of
Response Surface Methodology (RSM) to maximize bioethanol production. The suggested optimum conditions for
bioethanol production were agitation speed at 152 rpm and volume of OPFJ at 39.71% in which giving ethanol
concentration of 4.79 g/L. Growth profile after optimization indicated that the highest ethanol concentration (5.75%) was
achieved after 15 h of fermentation. Kinetic studies indicated that ethanol yield coefficient (Yp/s) due to consumption of
OPFJ and productivity of ethanol was 3.5 fold and 25% increased compared to before optimization, respectively. While,
in term of ethanol yield about 9% increased was observed.
Conclusion, Significance and Impact of study: This showed that OPFJ can be an alternative new feedstock for
bioethanol production using S. cerevisiae HC10.
- Full text:P020170105544744955837.pdf
