Studies on Skin Permeation in vitro of Phloretin and Ligustilide in Fufang Phloretin Cream
- VernacularTitle:复方根皮素乳膏中根皮素、藁本内酯体外透皮吸收研究
- Author:
Yang ZHANG
;
Qingwei WANG
;
Huanhuan ZHAO
;
Changjie LI
;
Yuan XU
- Keywords:
Cream;
Phloretin;
Ligustilide;
Transdermal absorption
- From:
China Pharmacist
2017;20(6):1038-1041
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the transdermal absorption of phloretin and ligustilide in Fufang phloretin cream.Methods: In vitro percutaneous absorption test using the Franz intelligent transdermal absorption apparatus was adopted, an HPLC method was used to determine the contents of phloretin and ligustilide in the accepting solution at different time points, and then the cumulative release amount and the total penetration amount were calculated to evaluate the percutaneous absorption behavior.Results: Phloretin and ligustilide showed a good linearity within the range of 0.044 2-4.42 μg·ml-1(r=0.999 9) and 0.048 7-4.87 μg·ml-1(r=0.999 9), respectively, the average recovery was 99.85% and 99.92%, and RSD was 0.67% and 0.66% (n=9), respectively.The in vitro permeation behavior of phloretin and ligustilide through the skin of rats fit Higuchi equations(r>0.9), suggesting a good linear correlation between the cumulatine penetration amount (Qn) and (time).Conclusion: Fufang phloretin cream has a good percutaneous permeation property in vitro.
