The Anti Tumor Effect of Hyaluronic Acid Decoreted Chlorogenic Acid Liposomes on Mice Bearing Uterine Cervical Carcinoma (U14)
10.13241/j.cnki.pmb.2017.26.006
- VernacularTitle:透明质酸修饰的绿原酸脂质体对U14宫颈癌荷瘤小鼠的抑制作用
- Author:
Yuxi ZHAO
;
Ying WEI
;
Zhen CHEN
;
Shipeng ZHANG
;
Fan ZHANG
- Keywords:
Hyaluronic acid;
Chlorogenic acid;
Target;
Liposomes;
Tumor
- From:Progress in Modern Biomedicine
2017;17(26):5029-5033
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the formulation of HA-CA liposomes and to study the anti-tumor effect of HA-CA liposomes on uterine cervical carcinoma mice.Methods:The methods of preparing HA-CA liposomes were screened,and the optimal fomulation was selected by the orthogonal design experiment with the the phospholipids/cholesterol ratio,the drug/lipids ratio and the pH value of PBS buffer was 7.4 as entrapment enfficiency was the index.The release of HA-CA liposomes was studyed by dialysis bag method.Uterine cervical carcinoma cells were inoculated subcutaneously into right axillary ofBal b/c mice,after continuous treatment of 14 d,we weighed the tumor and calculated the rate of tumor growth inhibition.Results:HA-CA liposomes were prepared by thin film hydration methods.The optimal parameters were as follows:the phospholipids/cholesterol ratio was 4∶1,the drug/lipids ratio was 1∶ 30,the pH value of PBS buffer was 7.4.The release curve of HA-CA liposome and CA liposome was basically the same,both of which a sustained-release efficacy.The cumulative release of HA-CA liposomes and CA liposomes were 78.39％ and 83.01％ at 48h.The inhibition rate of HA-CA liposomes on U14 cervical cancer mice was 60.39％ and significantly higher than that of positive control group,which was higher than that of CA and CA liposomes.Conclusions:HA-CA liposomes can significantly inhibit the effect of U14 cervical cancer nude mice higher than that of CA and CA liposomes owe to the modification of the active target ligand HA.
