Property and pharmacodynamics of uricase-catalase liposomes
10.3969/j.issn.1001-1978.2017.09.007
- VernacularTitle:尿酸酶-过氧化氢酶脂质体的特性分析及药效学研究
- Author:
Lan YANG
;
Yunli ZHOU
;
Yao LI
;
Shenglei YAN
;
Jingqing ZHANG
- Keywords:
uricase;
catalase;
liposome;
hyperuricemia;
mice;
pharmacodynamics;
optimal temperature and optimal pH
- From:
Chinese Pharmacological Bulletin
2017;33(9):1211-1214
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the optimal temperature and optimal pH of uricase-catalase liposomes(UCALP) and free uricase(UAE), and study the abilities of UCALP to reduce uric acid and hydrogen peroxide in mice with hyperuricemia.Methods UCALP were prepared by reverse phase evaporation, optimal temperature and optimal pH of UCALP and UAE were determined, respectively.Mouse model of hyperuricemia was established by intraperitoneally injection of uric acid, and the model mice were intravenously injected UCALP and UAE, respectively, then the serum concentration of uric acid and hydrogen peroxide in mice at different time points were measured by the assay kits, respectively.Results Optimal temperature of UCALP and UAE was 40℃, and optimal pH was 8.0 and 8.5, respectively.UCALP could more significantly lower uric acid level of hyperuricemia mice than that of UAE, and the concentration of hydrogen peroxide in UCALP group was lower than in UAE group.Conclusion UCALP can effectively decrease the level of uric acid and control the level of hydrogen peroxide in mice with hyperuricemia.
